Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 5. BMP gene expression in the pharyngeal pouches during epibranchial development, and defects in cas mutants. Lateral views, anterior to the left. Whole- mount in situ hybridization was performed in wild types at 22 hpf (top row), 30 hpf (second row) and 40 hpf (third row), and in cas mutants at 40 hpf (bottom row), for bmp2a (A,E,I,M), bmp2b (B,F,J,N), bmp4 (C,G,K,O) and bmp5 (D,H,L,P). Arrows indicate initial expression at the ventrolateral edges of each pouch. Arrow in H denotes bmp5 expression in the dorsal pouches. 1-5, pharyngeal pouches; ot, otic capsule.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: Gene Expression, In Situ Hybridization, Expressing

Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 8. BMP4-coated beads induce, and NOG-coated beads inhibit, epibranchial formation. Lateral views, anterior to the left. In situ hybridization for phox2b mRNA to label epibranchials at 48 hpf. (A) Wild-type control embryo. (B-F) Embryos that received Affi-Gel Blue beads soaked in either PBS (B), BMP4 (10 μg/ml, C; 1 μg/ml, D) or NOG protein (500 pg/ml, E; 300 pg/ml, F). Arrows in C and D indicate ectopic phox2b+ cells adjacent to the bead; arrows in E and F denote missing ganglia adjacent to the NOG-coated bead. Asterisks indicate beads. gVII, facial; gIX, glossopharyngeal; gX, vagal; ot, otic vesicle. Scale bar: 50 μm.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: In Situ Hybridization, Control

Journal: Stem cell research & therapy

Article Title: Centrifugal gravity-induced BMP4 induces chondrogenic differentiation of adipose-derived stem cells via SOX9 upregulation.

doi: 10.1186/s13287-016-0445-6

Figure Lengend Snippet: Fig. 3 CG induced SOX9 upregulation via increased expression BMP4. a Expression of BMP2, BMP4, and TGF-β1 mRNAs in CG-stimulated ASCs. ASCs were loaded with different degrees of CG (0, 300, 600, 1200, and 2400 g) for 15 min. b The duration of BMP4 mRNA expression in CG-stimulated ASCs. c Expression of BMP4 protein in CG-stimulated ASCs. ASCs were centrifuged at 2400 g for 30 min and then harvested at the indicated time points. Expression of BMP4 mRNA and protein was examined by RT-PCR and western blotting, respectively. d BMP4-dependent SOX9 expression in CG- stimulated ASCs. ASCs were stimulated with CG (2400 g for 30 min) and treated with recombinant BMP4 (10 nM) or Dorsomorphin (25 nM). SOX9 protein expression was examined using western blotting. The intensities of the bands in western blots were determined using ImageJ 1.40. BMP bone morphogenetic protein, CG centrifugal gravity, SOX SRY (sex-determining region Y)-box, TGF transforming growth factor

Article Snippet: Protein lysates (30 μg) and concentrated supernatants were resolved by 10–12% SDS-PAGE, transferred to polyvinylidene difluoride membranes (Amersham Pharmacia Biotech, Piscataway, NJ, USA), and then probed with primary antibodies against SOX9 (EMD Millipore, Billerica, MA, USA), BMP4 (Santa Cruz Biotechnology, Inc., Dallas, Texas USA), and GAPDH (Abcam).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Recombinant